Phytoplasma - nouveau livre

Livre dans la base de données depuis 2008-11-29T12:56:29+01:00 (Zurich)
Page de détail modifiée en dernier sur 2023-11-16T17:34:59+01:00 (Zurich)
ISBN/EAN: 1627030883

ISBN - Autres types d'écriture:
1-62703-088-3, 978-1-62703-088-5
Autres types d'écriture et termes associés:
Auteur du livre: von matt, hodge, dickinson
Titre du livre: dickinson

Données de l'éditeur

Auteur: Matt Dickinson; Jennifer Hodgetts
Titre: Methods in Molecular Biology; Phytoplasma - Methods and Protocols
Editeur: Humana; Humana Press
421 Pages
Date de parution: 2012-09-18
Totowa; NJ; US
Imprimé / Fabriqué en
Langue: Anglais
160,49 € (DE)
164,99 € (AT)
177,00 CHF (CH)
POD
XIII, 421 p.

BB; Hardcover, Softcover / Biologie/Botanik; Botanik und Pflanzenwissenschaften; Verstehen; phytoplasma diseases; phytoplasma genomes; plant DNA; plant pathology; taxonomic groups; Plant Science; Plant Genetics; Pflanzenbiologie; Genetik (nicht-medizinisch); EA; BC

series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors.

The Phytoplasmas - An Introduction.- Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus.- Micropropagation and Maintenance of Phytoplasmas in Tissue Culture.- Dodder Transmission of Phytoplasmas.- Insect Maintenance and Transmission.- Capturing Insect Vectors of Phytoplasmas.- Insect Vector Transmission Assays.- Molecular Identification of Phytoplasma Vector Species.- Dienes’ Staining and Light Microscopy for Phytoplasma Visualization.- DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas.- Visualisation of Phytoplasmas using Electron Microscopy.- Automated DNA Extraction for Large Numbers of Plant Samples.- DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts.- Nested PCR and RFLP Analysis Based on the 16S rRNA Gene.- PCR and RFLP Analyses Based on the Ribosomal Protein Operon.- Tuf and secY PCR Amplification and Genotyping of Phytoplasmas.- PCR Analysis of Phytoplasmas Based on the secA Gene.- Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains.- Microarrays for Universal Detection and Identification of Phytoplasmas.- T-RFLP for Detection and Identification of Phytoplasmas in Plants.- Real-Time PCR for Universal Phytoplasma Detection and Quantification.- A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target DNA.- Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group.- Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions.- In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP).- DNA Bar-Coding for Phytoplasma Identification.- Phylogenetic Analyses of Phytoplasmas Based on Whole-Genome Comparison.- The iPhyClassifier, An Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment.- Phytoplasma Proteomic Analysis.- Preparation ofPhytoplasma Membrane Recombinant Proteins.- Phytoplasma Plasmid DNA Extraction.- Caesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA.- Pulsed-Field Gel Elecrophoresis for Isolation of Full-Length Phytoplasma Chromosomes From Plants.- Mapping the Phytoplasma Chromosome.

series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors.