Reverse Chemical Genetics - Livres de poche

EAN (ISBN-13): 9781617796418
ISBN (ISBN-10): 1617796417
Version reliée
Livre de poche
Date de parution: 2014
Editeur: Humana
284 Pages
Poids: 0,596 kg
Langue: Englisch

Livre dans la base de données depuis 2007-07-19T13:14:31+02:00 (Zurich)
Page de détail modifiée en dernier sur 2023-07-29T05:33:48+02:00 (Zurich)
ISBN/EAN: 9781617796418

ISBN - Autres types d'écriture:
1-61779-641-7, 978-1-61779-641-8
Autres types d'écriture et termes associés:
Auteur du livre: hisashi
Titre du livre: molecular genetics

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Données de l'éditeur

Auteur: Hisashi Koga
Titre: Methods in Molecular Biology; Reverse Chemical Genetics - Methods and Protocols
Editeur: Humana; Humana Press
272 Pages
Date de parution: 2012-05-04
Totowa; NJ; US
Imprimé / Fabriqué en
Langue: Anglais
128,39 € (DE)
131,99 € (AT)
142,00 CHF (CH)
POD
XII, 272 p. 64 illus., 4 illus. in color.

BC; Hardcover, Softcover / Medizin/Klinische Fächer; Genetik, Medizin; Verstehen; 2D electrophoresis; Escherichia coli; In situ hybridization; Microarray; PCR; Small molecules; Surface plasmon resonance; Target genes; cDNA libraries; gene expression; genes; genetics; hybridization; molecular biology; Medical Genetics; BB

The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics.- ORFeome Cloning.- Systematic Cloning of an ORFeome Using the Gateway System.- High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins.- Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study.- High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data.- Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology.- Modification of cDNA Resources Utilized for Reverse Chemical Genetics.- High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources.- An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources.- Recombinant Protein Production by a Kaiko–Baculovirus System.- Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins.- The Refinement of Established Methods by Recent Innovations.- Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine.- Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis.- Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos.- Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins.- Potential Applicable Techniques for Reverse Chemical Genetics.- Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases.- Preparation of Highly Sensitive Protein Array Using Reactive Polymer.- High-Throughput SPR Biosensor.- Drug Discovery Through Functional Screening in the Drosophila Heart.- Permeable Cell Assay: A Method for High-ThroughputMeasurement of Cellular ATP Synthetic Activity.- Novel Methodology for Immobilization of Biomolecules on the Surface of a Photoresponsible Polymer Containing Azobenzene Moiety.
Orchestrates the differential fields of technology in order to create a new era of the study of reverse chemical genetics Introduces the fundamental resources and continues with the most recent molecular technologies Presents applicable techniques and functional assays utilizing specific small molecules